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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
14/03/2017 |
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Data da última atualização: |
14/03/2017 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
COTTA, M. G. |
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Afiliação: |
MICHELLE GUITTON COTTA. |
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Título: |
Molecular mechanisms in the first step of aba-mediated response in Coffea ssp. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
2016. |
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Páginas: |
169 p. |
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Idioma: |
Inglês |
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Notas: |
Tese (doutorado Biotecnologia Vegetal) - Universidade Federal de Lavras, Lavras, MG. Orientador: Alan Carvalho Andrade. Coorientador: Pierre Roger René Marraccini. |
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Conteúdo: |
Abscisic acid (ABA) pathway is a phytohormone universally conserved in land plants which coordinates several aspects of the plant response to water deficit such as root architecture, seed dormancy and regulation of stomatal closure. A mechanism of ABA signal transduction has been proposed, evolving intracellular ABA receptors (PYR/PYL/RCARs) interacting with PP2Cs phosphatases and SnRK2 protein kinases regulating this tripartite protein system. The goal of this study was to identify and characterize for the first time the orthologs genes of this tripartite system in Coffea. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, tomato and potato were chosen as query to search orthologous genes in the Coffee Genome Hub (http://coffee-genome.org/). Differential expression in tissues as leaves, seeds, roots and floral organs waschecked through in silico analyses. In vivo gene expression analyses were also performed by RT-qPCR in leaves and roots of drought-tolerant (DT 14, 73 and 120) and drought-susceptible (DS 22) C. canephora Conilon clones submitted (or not) to drought. The expression profiles of the tripartite system CcPYL-PP2C-SnRK2 genes were also analyzed in leaves of C. arabica and C. canephora plants growing under hydroponic condition and submitted to ABA exogenous treatment (500 ?M). This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCARs, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein motifs identified in predict coffee sequences enabled characterize these genes as family’s members of PYL/RCARs receptors, PP2Cs phosphatases or SnRK2 kinases of the ABA response pathway. These families were functionally annotated in the C. canephora genome. In vivo analyses revealed that eight genes are upregulated under drought conditions in both leaves and roots tissues. Among them, three genes coding phosphatases were expressed in all (DT and DS) clones therefore suggesting that they were activated as a general response to cope with drought stress. However, two other phosphatase coding genes were up-regulated only in the DT clones, suggesting that they constituted key-genes for drought tolerance in these clones. The DT clones also showed differential gene expression profiles for five other genes therefore reinforcing the idea that multiple biological mechanisms are involved drought tolerance in C. canephora. In response to exogenous ABA, 17 genes were expressed in leaves of C. canephora and C. arabica plants. Several genes were differentially expressed in the DT clone 14 either in control condition or after 24h ABA treatment. Under control conditions, five genes were higher expressed in the C. canephora and C. arabica DT plants. The kinase CcSnRK2.6 was highlighted as gene specifically expressed in the C. canephora plants (DT and DS) after 72h of ABA treatment. Overall, it was observed that ABA signaling pathway is delayed in the DS C. arabica Rubi. Those molecular evidences corroborated with microscopies analyses which showed that the DT clone 14 was more efficient to control stomatal closure than other coffee plants in response to ABA treatment. All these evidences will help us to identify the genetic determinism of drought tolerance through ABA pathway essential to obtain molecular markers that could be used in coffee breeding programs.. MenosAbscisic acid (ABA) pathway is a phytohormone universally conserved in land plants which coordinates several aspects of the plant response to water deficit such as root architecture, seed dormancy and regulation of stomatal closure. A mechanism of ABA signal transduction has been proposed, evolving intracellular ABA receptors (PYR/PYL/RCARs) interacting with PP2Cs phosphatases and SnRK2 protein kinases regulating this tripartite protein system. The goal of this study was to identify and characterize for the first time the orthologs genes of this tripartite system in Coffea. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, tomato and potato were chosen as query to search orthologous genes in the Coffee Genome Hub (http://coffee-genome.org/). Differential expression in tissues as leaves, seeds, roots and floral organs waschecked through in silico analyses. In vivo gene expression analyses were also performed by RT-qPCR in leaves and roots of drought-tolerant (DT 14, 73 and 120) and drought-susceptible (DS 22) C. canephora Conilon clones submitted (or not) to drought. The expression profiles of the tripartite system CcPYL-PP2C-SnRK2 genes were also analyzed in leaves of C. arabica and C. canephora plants growing under hydroponic condition and submitted to ABA exogenous treatment (500 ?M). This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCARs, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein motifs ide... Mostrar Tudo |
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Palavras-Chave: |
Déficit hídrico. |
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Thesagro: |
Ácido abscísico; Hormonio vegetal. |
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Thesaurus Nal: |
Abscisic acid; Coffea; Plant hormones. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04070nam a2200205 a 4500
001 2066980
005 2017-03-14
008 2016 bl uuuu m 00u1 u #d
100 1 $aCOTTA, M. G.
245 $aMolecular mechanisms in the first step of aba-mediated response in Coffea ssp.$h[electronic resource]
260 $a2016.$c2016
300 $a169 p.
500 $aTese (doutorado Biotecnologia Vegetal) - Universidade Federal de Lavras, Lavras, MG. Orientador: Alan Carvalho Andrade. Coorientador: Pierre Roger René Marraccini.
520 $aAbscisic acid (ABA) pathway is a phytohormone universally conserved in land plants which coordinates several aspects of the plant response to water deficit such as root architecture, seed dormancy and regulation of stomatal closure. A mechanism of ABA signal transduction has been proposed, evolving intracellular ABA receptors (PYR/PYL/RCARs) interacting with PP2Cs phosphatases and SnRK2 protein kinases regulating this tripartite protein system. The goal of this study was to identify and characterize for the first time the orthologs genes of this tripartite system in Coffea. For this purpose, protein sequences from Arabidopsis, citrus, rice, grape, tomato and potato were chosen as query to search orthologous genes in the Coffee Genome Hub (http://coffee-genome.org/). Differential expression in tissues as leaves, seeds, roots and floral organs waschecked through in silico analyses. In vivo gene expression analyses were also performed by RT-qPCR in leaves and roots of drought-tolerant (DT 14, 73 and 120) and drought-susceptible (DS 22) C. canephora Conilon clones submitted (or not) to drought. The expression profiles of the tripartite system CcPYL-PP2C-SnRK2 genes were also analyzed in leaves of C. arabica and C. canephora plants growing under hydroponic condition and submitted to ABA exogenous treatment (500 ?M). This approach allowed the identification and characterization of 24 candidate genes (9 PYL/RCARs, 6 PP2Cs and 9 SnRK2s) in C. canephora genome. The protein motifs identified in predict coffee sequences enabled characterize these genes as family’s members of PYL/RCARs receptors, PP2Cs phosphatases or SnRK2 kinases of the ABA response pathway. These families were functionally annotated in the C. canephora genome. In vivo analyses revealed that eight genes are upregulated under drought conditions in both leaves and roots tissues. Among them, three genes coding phosphatases were expressed in all (DT and DS) clones therefore suggesting that they were activated as a general response to cope with drought stress. However, two other phosphatase coding genes were up-regulated only in the DT clones, suggesting that they constituted key-genes for drought tolerance in these clones. The DT clones also showed differential gene expression profiles for five other genes therefore reinforcing the idea that multiple biological mechanisms are involved drought tolerance in C. canephora. In response to exogenous ABA, 17 genes were expressed in leaves of C. canephora and C. arabica plants. Several genes were differentially expressed in the DT clone 14 either in control condition or after 24h ABA treatment. Under control conditions, five genes were higher expressed in the C. canephora and C. arabica DT plants. The kinase CcSnRK2.6 was highlighted as gene specifically expressed in the C. canephora plants (DT and DS) after 72h of ABA treatment. Overall, it was observed that ABA signaling pathway is delayed in the DS C. arabica Rubi. Those molecular evidences corroborated with microscopies analyses which showed that the DT clone 14 was more efficient to control stomatal closure than other coffee plants in response to ABA treatment. All these evidences will help us to identify the genetic determinism of drought tolerance through ABA pathway essential to obtain molecular markers that could be used in coffee breeding programs..
650 $aAbscisic acid
650 $aCoffea
650 $aPlant hormones
650 $aÁcido abscísico
650 $aHormonio vegetal
653 $aDéficit hídrico
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Registro original: |
Embrapa Café (CNPCa) |
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Biblioteca |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
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URL |
Voltar
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| Registros recuperados : 49 | |
| 4. |  | COTTA, M. G.; VERAS, H. C. T.; BARROS, L. M. G.; ABDELNOOR, R. V.; ALMEIDA, J. D.; SILVA, F. R. Finding novel tissue-specific genes using the new publically available soybean est database. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia. Charles Darwin: a origem das espécies: o livro que transformou a humanidade. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 86.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
|    |
| 5. | | COTTA, M. G.; MARRACCINI, P.; REGO, E. C. S.; SIDIBE-BOCS, S.; CARNEIRO, F. A.; THIS, D.; ANDRADE, A. C. First step of ABA-mediated response to drought in Coffea. In: In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls, Brazil. Book of Abstracts... Rockville, USA: American Society of Plant Biologists, 2015. p. 0881.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | SOUSA, H. R.; COTTA, M. G.; RODRIGUES, G. C.; ALMEIDA, J. D.; CARNEIRO, M.; BARROS, L. M. G. Isolamento de RNA de diferentes órgãos de Coffea arabica adequado para ensaios de expressão gênica. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 045. p. 85.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | COTTA, M. G.; BARROS, L. M. G.; ALMEIDA, J. D. de; ANDRADE, A. C.; CARNEIRO, M.; MARRACCINI, P. Validation of coffee seed promoter in nicotiana tabacum. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 256.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | BARROS, L. M. G.; ANDRADE, L. R. M. de; GEEST, J. J. Van D.; COTTA, M. G.; RIBEIRO, D. G.; FRANCO, A. C.; BECQUER, T. Acumulação de alumínio em tecidos foliares de espécies nativas do Cerrado. Brazilian Journal of Plant Physiology, Campinas, v. 19, 2007. Suplemento. 1 CD-ROM. Edição dos Resumos do XI Congresso Brasileiro de Fisiologia Vegetal, Gramado, set. 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 9. | | ANDRADE, L. R. M. de; BARROS, L. M. G.; ECHEVARRIA, G. F.; AMARAL, L. I. V. do; COTTA, M. G.; ROSSATTO, D. R.; HARIDASAN, M.; FRANCO, A. C. Al-hyperaccumulator Vochysiaceae from the Brazilian Cerrado store aluminum in their chloroplasts without apparent damage. Environmental and Experimental Botany, v. 70, p. 37-42, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | ANDRADE, L. R. M. de; BARROS, L. M. G.; ECHEVARRIA, G. F.; AMARAL, L. I. V.; COTTA, M. G.; ROSSATO, D. R.; HARIDASAN, M.; FRANCO, A. C. Al-hyperaccumulator vochysiaceae from the Brazilian Cerrado store aluminum in their chloroplasts without apprent damage. Environmental and experimental botany, v. 70, p. 37-42, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | COSTA, T. S.; CARNEIRO, F. A.; COTTA, M. G.; LIMA, E. A.; RÊGO, E. C. S.; MARRACCINI, P.; ANDRADE, A. C. Analysis of the expression of mannose-6-phosphate reductase gene in roots of different clones of C. canephora subbmited to water deficit. In: INTERNATIONAL CONFERENCE ON COFFE SCIENCE, 26., 2016, China. The programm & abstrcets... China: Asic, 2016. p. 186.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 12. | | ANDRADE, L. R. M. de; FRANCO, A. C.; HARIDASAN, M.; BARROS, L. M. G.; GEEST, J. J. Van D.; COTTA, M. G.; RIBEIRO, D. G. Alumínio nos ápices radiculares de espécies nativas do Cerrado. Brazilian Journal of Plant Physiology, Campinas, v. 19, 2007. Suplemento. 1 CD-ROM. Edição dos Resumos do XI Congresso Brasileiro de Fisiologia Vegetal, Gramado, set. 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 14. | | GEEST, J. J. V. D.; COTTA, M. G.; ANDRADE, L. R. M. de; BARROS, L. M. G.; ALMEIDA, J. D. de; FRANCO, A. C. Desenvolvimento de metodologia para detecção de alumínio nos tecidos de plantas do cerrado. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 3., 2007, Planaltina, DF. Resumos apresentados. Planaltina, DF: Embrapa Cerrados, 2007. p. 15. (Embrapa Cerrados. Documentos, 176).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | GEEST, J. J. V. D.; COTTA, M. G.; ANDRADE, L. R. M. de; BARROS, L. M. G.; ALMEIDA, J. D. de; FRANCO, A. C. Desenvolvimento de metodologia para detecção de alumínio nos tecidos de plantas do cerrado. In: ENCONTRO DE JOVENS TALENTOS DA EMBRAPA CERRADOS, 3., 2007, Planaltina, DF. Resumos apresentados. Planaltina, DF: Embrapa Cerrados, 2007. p. 15. (Embrapa Cerrados. Documentos, 176).| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Cerrados. |
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| 16. | | LIMA, E. A. de; REGO, E. C. S.; COTTA, M. G.; COSTA, T. S.; CARNEIRO, F. A.; CARNEIRO, R. M. D. G.; MARRACCINI, P.; ANDRADE, A. C. Differential expression of candidate genes to resistance Tomeloidogyne paranaensis in clones of Coffea canephora. In: INTERNATIONAL CONFERENCE ON COFFE SCIENCE, 26., 2016, China. The programm & abstrcets... China: Asic, 2016. p. 185.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 17. | | COSTA, T. S.; MELO, J. A. T.; CARNEIRO, F. A.; VIEIRA, N. G.; RÊGO, E. C. S.; LIMA, E. A.; COTTA, M. G.; MARRACCINI, P.; ANDRADE, A. C. Expression of candidates genes for drought tolerance related to ABA signaling in roots of C. Canephora. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 6., 2015, Brasília. [Resumos]. Brasília, DF: Sociedade Brasileira de Biotecnologia, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | COTTA, M. G.; BOCS, S.; REGO, E. C. S.; COSTA, T. S.; AQUINO, S. O. de; CARNEIRO, F. De A.; THIS, D.; MARRACCINI, P.; ANDRADE, A. C. Estudo das famílias gênicas CcPYL, CcPP2C e CcSnRK2: componentes centrais na via aba-dependente para a resposta ao déficit hídrico em Coffea Canephora. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 9., Curitiba. Brasília, DF: Embrapa Café, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 19. | | COTTA, M. G.; VERAS, H C. T.; BARROS, L. M. G.; ABDELNOOR, R. V.; ALMEIDA, J. D. D.; SILVA, F. R. de. Finding novel tissue-specific genes using the new publically available soybean est database. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 86.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | COTTA, M. G.; RÊGO, E. C. S.; BOCS, S. S.; COSTA, T. S.; DUFAYARD, J-F.; THIS, DOMINIQUE; MARRACCNINI, P.; ANDRADE, A. C. The first step of ABA perception and signal transduction in coffe: evolutionary and expressionof PYR/PYL/RCARs, PP2Cs and SnRK2s genes in C canephora under drought. In: INTERNATIONAL CONFERENCE ON COFFE SCIENCE, 26., 2016, China. The programm & abstrcets... China: Asic, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 49 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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